LOCALIZATION OF PHOSPHATIDIC ACID PHOSPHATASE ACTIVITY IN GRANULAR PNEUMONOCYTES
نویسندگان
چکیده
منابع مشابه
Localization of Phosphatidic Acid Phosphatase Activity in Granular Pneumonocytes
The alveoli of the mammalian lung are lined by a film of surface-active material (surfactant) which is commonly believed to be a phospholipid (1, 5, 8, 11) . A considerable body of evidence has accumulated which suggests that surfactant is synthesized by the granular pneumonocytes (type II cells) of the alveolar epithelium (1, 9, 10) . Although a number of acid hydrolases have been demonstrated...
متن کاملCharacteristics and subcellular localization of phospholipase d and phosphatidic Acid phosphatase in mung bean cotyledons.
Mixed micelles of (32)P-labeled phosphatidylcholine or phosphatidic acid (PA) and the nonionic detergent octylphenol polyethylene oxide (NP-40 Nonidet) were used to assay the activities of phospholipase D and PA phosphatase in crude extracts of mung bean (Vigna radiata) cotyledons. Together these enzymes degrade phosphatidylcholine to free choline, inorganic phosphate, and sn-1,2-diacylglycerol...
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The acid phosphatase activity of rat liver homogenates and of nuclear and cytoplasmic fractions isolated therefrom (by differential centrifugation) was determined biochemically in a series of experiments. For each liver, Gomori's histochemical test for acid phosphatase was run in parallel. No correlation was found between the biochemical and the histochemical results. According to the former, t...
متن کاملPulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase.
The lung contains two distinct forms of phosphatidic acid phosphatase (PAP). PAP1 is a cytosolic enzyme that is activated through fatty acid-induced translocation to the endoplasmic reticulum, where it converts phosphatidic acid (PA) to diacylglycerol (DAG) for the biosynthesis of phospholipids and neutral lipids. PAP1 is Mg(2+) dependent and sulfhydryl reagent sensitive. PAP2 is a six-transmem...
متن کاملPhosphorylation of lipin 1 and charge on the phosphatidic acid head group control its phosphatidic acid phosphatase activity and membrane association.
The lipin gene family encodes a class of Mg(2+)-dependent phosphatidic acid phosphatases involved in the de novo synthesis of phospholipids and triglycerides. Unlike other enzymes in the Kennedy pathway, lipins are not integral membrane proteins, and they need to translocate from the cytosol to intracellular membranes to participate in glycerolipid synthesis. The movement of lipin 1 within the ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.53.1.249